Diagnostic significance of TLR2 and TLR4 receptors on lymphoid cells as a marker of the progression of periodontal inflammation associated with key periodontal pathogenic species <i>F. alocis</i> and <i>P. gingivalis</i>

The aim of the work was to evaluate diagnostic value TLR2 and TLR4 expression on periodontal peripheral blood lymphoid cells by immunofluorescence microscopy in patients with chronic periodontitis associated key pathogenic species Filifactor alocis, Porphyromonas gingivalis.
 Materials methods. study included 150 88 (59%) women 62 (41%) men aged 18 73 years acute phase (CP) 32 people without signs inflammation. To confirm diagnosis periodontitis, Multident-5 PCR kit used (detection P. gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitans), as well rt-PCR for F. alocis gingivalis contents pocket (NPF GenLab, Russia). carrying CD282 CD284 markers, gingival fluid flushes from Hanks' solution were used. isolated stained antibodies markers (corresponding receptor) or labeled FITC, fixed paraformaldehyde subsequent microscopy.
 Results. leukocytes studied individuals healthy gingivalis. According results PCR, detection rate 64 62.7%, respectively, which confirmed their dominance microbial association. It found that varied humans. possible significance this phenomenon assessing progression is discussed.
 Conclusion. In periodontopathogenic multidirectional observed, may have diseases.